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Kelly Servage

Kelly Servage

My current research in Dr. David Russell’s Laboratory, Professor, Department of Chemistry and Director of the Laboratory for Biological Mass Spectrometry (LBMS), focuses on the development and utilization of cryogenic ion mobility-mass spectrometry (cryo-IM-MS) towards an understanding of the environment-dependent structure of biological molecules, i.e. peptides and proteins. Experimental conditions reduce ion heating on the timescale of the experiment and facilitate ion transfer from solution to the gas phase with minimal perturbation of the solution structure. We have shown evidence for this recently through the kinetic trapping of a compact population of conformers for the peptide Substance P, as well as, the preservation of protonated water clusters and elucidation of the structural transitions that occur upon the development of the H-bond network. The collaboration involving Dr. Peter Stang, 2013-2014 TIAS Fellow and Professor, Department of Chemistry, University of Utah, and Dr. Russell focuses on the development of novel MS and IM-MS methods for the characterization of macromolecules that are synthesized by Dr. Stang’s research group. The ability to characterize these molecules using typical ESI-MS and ESI-IM-MS techniques presents difficulties because source conditions are typically too hot to preserve the intact structures of these large molecules for analysis. However, novel instrumentation techniques that provide cool conditions, especially cryo-IM-MS, minimize, or possibly eliminate, the thermal degradation of these molecules as they are transferred from solution to the gas phase. We are currently in the process of developing new instrumentation, including the incorporation of an ion mobility drift cell onto the Thermo Scientific Exactive Plus EMR (extended mass range) “Orbitrap.” The proposed instrument will merge high resolution drift cell-ion mobility with macromolecule mass spectrometry to provide greater versatility for performing conformer-specific structural characterization on large molecules. The development and utilization of home-built instrumentation provides new approaches for addressing complex structural biology questions by retention of solution-phase structures of large molecules upon transition to the gas phase.

Major

Chemistry

Graduation Year

August 2016

Degree Type

Ph.D.

Fellowship Year(s)

'13 - '14

Previous Education

B.S. in Chemistry - St. Edward's University - '12

Advisor

D. Russell

Journal Articles

1. Servage, K. A.; Silveira, J. A.; Fort, K. L.; Russell, D. H. Cryogenic Ion Mobility-Mass Spectrometry: Tracking Ion Structure from Solution to the Gas Phase. Acc. Chem. Res. 2016, 49, 1421-1428.

2. Servage, K. A.; Silveira, J. A.; Fort, K. L.; Clemmer, D. E.; Russell, D. H. Water-Mediated Dimerization of Ubiquitin Ions Captured by Cryogenic Ion Mobility-Mass Spectrometry. J. Phys. Chem. Lett. 2015, 6, 4947-4951.

3. Servage, K. A.; Fort, K. L.; Silveira, J. A.; Shi, L.; Clemmer, D. E.; Russell, D. H. Unfolding of Hydrated Alkyl Diammonium Cations Revealed by Cryogenic Ion Mobility-Mass Spectrometry. J. Am. Chem. Soc. 2015, 137, 8916-8919.

4. Servage, K. A.; Silveira, J. A.; Fort, K. L.; Russell, D. H. From Solution to Gas Phase: The Implications on the Evaporative Dynamics of Substance P During Electrospray Ionization. J. Phys. Chem. B 2015, 119, 4693-4698.

5. Servage, K. A.; Silveira, J. A.; Fort, K. L; Russell, D. H. Evolution of Hydrogen-Bond Networks in Protonated Water Clusters H+(H2O)n (n = 1 to 120) Studied by Cryogenic Ion Mobility-Mass Spectrometry. J. Phys. Chem. Lett. 2014, 5, 1825-1830.

6. Fort, K. L.; Silveira, J. A.; Pierson, N. A.; Servage, K. A.; Clemmer, D. E.; Russell, D. H. From Solution to the Gas Phase: Factors that Influence Kinetic Trapping of Substance P in the Gas Phase. J. Phys. Chem. B 2014, 118, 14336-14344.

7. Silveira, J. A.; Fort, K. L.; Kim, D.; Servage, K. A.; Pierson, N. A.; Clemmer, D. E.; Russell, D. H. From Solution to the Gas Phase: Stepwise Dehydration and Kinetic Trapping of Substance P Reveals the Origin of Peptide Conformations. J. Am. Chem. Soc. 2013, 135, 19147-19153.

8. Silveira, J. A.; Servage, K. A.; Gamage, C. M.; Russell, D. H. Cryogenic Ion Mobility-Mass Spectrometry Captures Hydrated Ions Produced During Electrospray Ionization. J. Phys. Chem. A 2013, 117, 953-961.

Presentations

1. Servage, K. A.; Russell, D. H. (2016). Water-Mediated Dimerization of Ubiquitin Ions Captured by Cryogenic Ion Mobility-Mass Spectrometry. Poster Presentation, 64th ASMS Conference on Mass Spectrometry and Allied Topics, San Antonio, TX.

2. Servage, K. A.; Fort, K. L.; Silveira, J. A.; Shi, L.; Clemmer, D. E.; Russell, D. H. (2015). The Role of Inter- and Intramolecular Interactions on Conformational Preferences During Electrospray Ionization: Unfolding of Alkyl Diammonium Cations. Poster Presentation, 63rd ASMS Conference on Mass Spectrometry and Allied Topics, St. Louis, MO.

3. Servage, K. A.; Silveira, J. A.; Fort, K. L.; Russell, D. H. (2015). From Solution to the Gas Phase: The Implications of Intramolecular Interactions on the Evaporative Dynamics of Substance P During Electrospray Ionization. Poster Presentation, Gordon Research Conference; Gaseous Ions: Structures, Energetics, and Reactions, Galveston, TX.

4. Servage, K. A.; Silveira, J. A.; Fort, K. L.; Russell, D. H. (2014). Evolution of Hydrogen-Bond Networks in Protonated Water Clusters H+(H2O)n (n = 1 to 120) Studied by Cryogenic Ion Mobility-Mass Spectrometry. Poster Presentation, 62nd ASMS Conference on Mass Spectrometry and Allied Topics, Baltimore, MD.